860 Gitts Run Road
Hanover, PA 17331

Enzymes and the effect of pH 8-104

Target Grades: 10-14

This kit demonstrates the effect of pH on enzyme activity using the enzymes Invertase and Amylase. The pH affects the activity of enzymes in two ways: by affecting the charges on groups in the enzyme’s three-dimensional active site and by altering the charges which hold the enzyme molecule together. Altering the charges in the active site can greatly change enzyme activity, but these changes are usually reversible. When an enzyme molecule is denatured by pH changes, the effects are usually not reversible and the enzyme becomes permanently inactive.  Enzyme activity is measured using DNS reduction to ANS, which may be assessed visually or by spectrophotometer. Required, but not included: lab glassware; including beakers, test tubes, and graduated cylinders; a hot plate or water bath, use of a spectrophotometer or colorimeter is optional (absorption 540 nm). Reply card must be returned to obtain Invertase and Amylase enzymes. Reply card items are only shipped within the continental United States. Designed for advanced biology classes and includes sufficient chemicals for 24 students working in six groups of four.

Kit Contains:
Instructor Manual
Student Data Sheets
Assessment Manual
pH paper 1-14
Amylase Solution
Invertase Solution
Iodine Solution
Buffer 1 Solution
Buffer 2 Solution
Buffer 3 Solution
Sodium Hydroxide
Hydrochloric Acid
3,5 Dinitrosalicylic Acid (DNS) Solution