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Enzymology, Advanced Kit 8-130

Target Grades: 11-14

The enzyme Invertase hydrolyzes sucrose into glucose and fructose. The amount of these reducing sugars can be measured with the chemical DNS which creates a distinctive color change. Students use a spectrophotometer to determine the reaction rate of Invertase when subjected to varied substrate concentrations, competitive and non-competitive inhibitors, changes in pH, and changes in temperature. Kit includes all necessary chemicals and a reply card for the Invertase enzyme and glucose solutions to be shipped separately upon request.  Reply card items are only shipped within the continental United States. Required, but not included: lab glassware; including beakers, test tubes, and graduated cylinders; a hot plate, and a spectrophotometer. Designed for advanced biology classes and includes sufficient chemicals
for 24 students working in six groups of four.

Kit Contains:
Instructor Manual
Student Data Sheets
MSDS
Buffer pH 3 Solution
Buffer pH 4 Solution
Buffer pH 5 Solution
Buffer pH 6 Solution
Buffer pH 7 Solution
Buffer pH 8 Solution
Buffer pH 9 Solution
Buffer pH 10 Solution
Buffer Substrate Effects Solution
Buffer Temperature Effects Solution
Buffer Inhibition Solution
Inhibitor Solution
Sucrose Inhibitor
Sucrose Substrate Effecis
Sucrose Temperature Effects
Sucrose pH Substrate
3,5 Dinitrosalicylic Solution (DNS)
Aniline Solution
Invertase Solution
Standard Glucose Solution

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