Target Grades: 9-12
Plant propagation by cloning is becoming increasingly important in food and flower production. Generating a plant by cloning its tissue is an exciting and challenging experience which teaches the importance of sterile laboratory methods. Students will germinate carrot seeds to provide plant material from which to establish a culture, use sterile technique to grow seedlings and establish a callus culture, and induce the development of multiple shoots (plantlets) from the callus tissue. The process takes approximately four months. No hood or incubator required. Includes an instruction manual, assessment questions, and a PowerPoint presentation with images of plant material at various stages of the experiment. Perishable reply card items ship separately upon request and only in the continental US.
Petri Dishes, shallow
Petri Dishes, deep
Reply Card for Murashige & Skoog Basal Medium with Vitamins, Carrot Callus Initiation Basal Medium, Plant Preservation Mixture (PPM)