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Sequence of a Peptide 8-160

Target Grades: College Level

Students will determine the amino acid sequence of a tripeptide isolated from a protein hydrolysate. The experiments in this kit enable students to determine the chain length of the peptide by formol titration, determine the amino acid content and composition of the tripeptide using chromatographic techniques to determine Rf values, and identify which amino acids are the N-terminal and C-terminal residues using solvent extraction, enzymatic digestion, and chromatographic techniques. This kit contains formaldehyde. Includes student data sheets. Designed for 24 students working in groups of four.

Kit Contains:
Capillary Tubes, Melting Point
Chromatography Paper Strips
Pump Spray Bottle
Vials, 8 Dram Shell, Glass
L-Proline Standard
Phenylalanine Standard
Arginine Standard
Glycine Standard
Lysine Standard
DNP-Peptide Derivative
DNP-Phenylalanine
DNP-Glycine
Hydrolyzed Peptide
Unhydrolyzed Peptide
Carboxypeptidase, Hydrolyzed
Butanol
Acetone
Formaldehyde, 37%
Sodium Hydroxide
Ninhydrin
Ethanol - Ammonia Solution
Phenolphthalein

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